粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究   总被引：6，自引：0，他引：6  

郝林华  陈靠山  牛德庆  张玉凤 《海洋科学进展》2006,24(1):66-73

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。  相似文献   

南海晚第四纪硅藻遗体及其地质意义：Ⅷ.几个问题的讨论     

蓝东兆  程兆第 《台湾海峡》1995,14(3):235-240

本文根据分析结果阐述了两种不同样品处理方法所获得的硅藻丰度和微型硅藻的人主要种类和相对百分含量。研究表明洗涤富集法所获得的硅藻丰度存在较大误差；微型硅藻的含量普遍介于１０％－２０％之间，最高可达４２．３１％，最低为５．６６％，并指出了在微型硅藻相对含量大于２０％的样品中 ，其主要种类为具槽直链藻和条纹小环藻等沿岸底格格不入中，并成群体产出。  相似文献   

Fully nonlinear numerical wave tank (NWT) simulations and wave run-up prediction around 3-D structures     

J. -C. Park  M. -H. Kim  H. Miyata  H. -H. Chun 《Ocean Engineering》2003,30(15):1969-1996

A finite-difference scheme and a modified marker-and-cell (MAC) algorithm have been developed to investigate the interactions of fully nonlinear waves with two- or three-dimensional structures of arbitrary shape. The Navier–Stokes (NS) and continuity equations are solved in the computational domain and the boundary values are updated at each time step by the finite-difference time-marching scheme in the framework of a rectangular coordinate system. The fully nonlinear kinematic free-surface condition is implemented by the marker-density function (MDF) technique developed for two fluid layers.To demonstrate the capability and accuracy of the present method, the numerical simulation of backstep flows with free-surface, and the numerical tests of the MDF technique with limit functions are conducted. The 3D program was then applied to nonlinear wave interactions with conical gravity platforms of circular and octagonal cross-sections. The numerical prediction of maximum wave run-up on arctic structures is compared with the prediction of the Shore Protection Manual (SPM) method and those of linear and second-order diffraction analyses based on potential theory and boundary element method (BEM). Through this comparison, the effects of non-linearity and viscosity on wave loading and run-up are discussed.  相似文献   

Experimental studies on the slowly varying drift motion of a berthed container ship model     

S.K. Lee  H. Choi  S. Surendran 《Ocean Engineering》2006,33(17-18):2454-2465

The effects of free-surface waves on the floating structures are of great importance in the offshore industry. Among the six degrees of motions of a surface ship the absence of restoring forces in surge, sway and yaw led to critical situations for moored ships in the recent times. The order of forces in horizontal plane and their exciting frequencies are matters of interest. The resonance with the presence of moored chains led to many accidents in the recent past. The lines in dry conditions may not give good damping and in wet condition they may trigger the system to chaotic motions and jumps. Two different loading conditions of a container ship model are tested with waves in laboratory conditions in two different drafts. The mooring lines are chosen as per scale law and the energy under the response spectrum is determined from the plots. The results give new insights into the movement of a berthed ships subjected to waves. Response of the moored ship to different loading conditions in different water depths are discussed in this paper. The paper gives the order of energy due to first-order and slowly varying movement of a berthed container model in a towing tank.  相似文献   

Effect of strong winds on the nutritional condition of anchovy (Engraulis encrasicolus L.) larvae in the Bay of Biscay, Northeast Atlantic, as inferred from an early field application of the DNA/C index     

Bergeron  Jean-Pierre 《ICES Journal of Marine Science》2000,57(2):249-255

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莱州湾东部滨海砂金成矿远景初步分析     

刘洪树  姜玉池 《海洋地质与第四纪地质》1991,11(2):17-24

本文阐述了莱州湾东部滨海砂金的物源、地貌、水动力、第四系、新构造运动和海平面变化等成矿条件以及砂金的富集特点,对该区砂金的成矿远景进行了区划和评价,初步认为该区具有良好的砂金成矿远景。  相似文献   

中国龙虾生长特点及促生长途径的探讨   总被引：1，自引：0，他引：1  

陈政强  陈昌生  黄永春  黄志灵 《海洋科学》2006,30(12):25-31

蜕壳后新甲壳完全硬化之前是中国龙虾(Panulirus stimpsoni)个体蜕壳生长过程的延续,对中国龙虾正常快速生长具有重要意义。环境因子、营养条件对中国龙虾的蜕壳与生长具有显著影响。24-27℃是中国龙虾蜕壳生长的适温范围;盐度24-32适宜中国龙虾生长,最适生长盐度是28;在6种试验饵料中翡翠贻贝饲养中国龙虾效果最好,其次是杂虾、杂蟹、牡蛎,投喂杂鱼的中国龙虾蜕壳增长率和阶段生长率都很低;龙虾饲料中添加蜕壳素能够縮短蜕壳周期,实现蜕壳率和蜕壳增长率的同步增长。摘除眼柄也能促进中国龙虾的蜕壳与生长,电刺激等外源刺激则只能促进蜕壳,而不能加速中国龙虾的生长,甚至可能产生负面影响。中国龙虾生长存在种内性别间的差异,同等条件下雄性个体的蜕壳增长率高于雌性个体。  相似文献   

柴达木盆地新构造运动主要特征与成因机制   总被引：10，自引：0，他引：10  

杨明慧 《海洋地质与第四纪地质》1997,17(3):71-78

研究表明，在壳幔结构、地球物理背景、沉积物结构、多层关地幔和构造样式等方面，达木盆地与中国东部新生代裂谷类伸展盆地具有可比性，应该归属 展性盆 。盆地伸展和收缩作用相间进行，伸展起因于地幔底辟；而收缩受两人因素影响；（１）盆地伸展作用受相邻造山带反作用诱导的挤压应力；（２）板块碰撞传递的远程板内应力。在此基础邮新的构造期达木盆地演化的“双层模式”，即地幔上隆－地壳伸展；被动挤压＋板内应力－地壳收缩  相似文献   

我国东部邻海恶劣天气的时空分布及其成因初探   总被引：2，自引：0，他引：2  

陈乾金  王伯民 《海洋预报》1993,10(1):10-19

本文利用1930～1979年NCDC海洋船舶观测的气象资料,分析了中国东部邻海恶劣天气的时空分布。结果表明,海区恶劣天气的分布在冬季和夏季是不同的;在东北和西南季风期间,台湾东北和西南海区也有差异。影响恶劣天气条件的分布因子非常复杂,主要是季风和极锋、天气系统、冷暖洋流及地形等因子。  相似文献   

海水中A3钢样阴极保护电化学场的二维有限元计算     

王在峰  孙虎元  孙立娟 《海洋科学》2006,30(7):38-42

有限元法已被证明是计算阴极保护场中电位、电流密度分布的有效方法。作者建立了二维的有限元数学模型,并讨论了模型中电化学场的非线性边界条件的处理和非线性方程组的迭代法解法。用构建的模型对简单的阴极保护模拟体系进行了分析,得出了较合理的结果。  相似文献